Non-immortalized somatic stem cell line derived from human umbilical cord blood

Biotechnological Hub of the NIB (BTH-NIB)

The purpose of the investment project BTH-NIB is the assurance of the appropriate infrastructural conditions for the use of research and developmental opportunities in the fields of operation of the NIB.

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Official opening of the Biotechnological Hub of the National Institute of Biology and the presentation of the Zei Awards.

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Construction of the Biotechnological Hub of the NIB is complete

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Services and products

Agriculture Industry Services

National Institute of Biology is authorized by the Ministry of Agriculture and the Environment to perform defined tasks of public service for the protection of plants.

Environmental Monitoring and Water-quality Testing Services

The principles of sustainable development and modern environmental protection policy require systematic monitoring of the status of nature and human environment.

Pharmaceutical and Biotechnology Industry Services

We offer bioanalytical laboratory support, toxicology studies, genetic toxicology testing of different samples, residual DNA detection, improvements of production lines through systems biology, and cotoxicology studies.

Research equipment

The big research equipment is used as support to our research and educational activities and serves technological development in co-operation with commercial enterprises.

Training and Related Services

We offer hands on training, consulting and technical auditing, development of protocols and workflows, organization of workshops.

Mutagenicity analysis under GLP

On the NIB we have certified GLP laboratory for testing mutagenicity with bacterial reverse mutation assay, or the Ames test (OECD 471).

25 Mar
Non-immortalized somatic stem cell line derived from human umbilical cord blood

25.03.2008, Nacionalni Inštitut za Biologijo, Biološko središče, Predavalnica B4 at 13:00 Leonora Buzanska ECVAM, IHCP, JRC, European Commission, Ispra, Italy; NeuroRepair Dept, Medical Research Center, Polish Academy of Science, Warsaw, Poland: Non-immortalized somatic stem cell line derived from human umbilical cord blood
A population of stem/progenitor cells was selected from human cord blood non-hematopoietic (CD34 and CD45 negative) mononuclear fraction and was shown to attain neural features. Due to repeated expansion and selection of these cells in the presence of EGF we have established the first clonogenic, non-immortalized human umbilical cord blood neural stem cell line (HUCB-NSC).This cell line can be maintained in culture at different developmental stages and their fate decisions can be experimentally manipulated in vitro by the presence of trophic factors, mitogenes and neuromorphogenes. Standardized conditions for their growth and differentiation have been established.