Monitoring of inland waters - Comet

Biotechnological Hub of the NIB (BTH-NIB)

The purpose of the investment project BTH-NIB is the assurance of the appropriate infrastructural conditions for the use of research and developmental opportunities in the fields of operation of the NIB.

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Monitoring of inland waters - Comet

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Single cell gel electrophoresis or comet assay is a rapid and sensitive method for detection of DNA single-strand breaks and double-strand breaks, alkali labile sites, which are the consequence of direct DNA damage or they occur as intermediates formed during the repair of DNA lesions. The method is appropriate for testing of the ability of different compounds and environmental samples to induce DNA damage. Comet assay can be performed on several cell lines (e.g. human hepatoma cell line HepG2, Chinese hamster ovarian cells CHO, human lymphocytes etc.) at different testing conditions, depending on the purpose of the test.

Contact: Assist. Prof. Dr. Bojana Žegura