VABILO

Nacionalni inštitut za biologijo Vas vabi, da se udeležite seminarja

»ACGT cis-regulatory elements in Plant gene expression: PP2C and AtAVT6D promoter as a case study«,

ki ga bo predstavil

prof. Rajesh Mehrotra, PhD
profesor na Oddelku za biologijo in prodekan za mednarodno sodelovanje na Birla Institute of Technology & Science (Goa, India).

Seminar bo v ponedeljek, 26. junija 2023, ob 11. uri
v Biološkem središču, Večna pot 111, v Ljubljani, v predavalnici B3.

Abstract:
The transcription control is achieved by interactions between cis-regulatory elements and transcription factors which are central to the gene expression process. The ACGT core sequence has been established as a functionally important cis element in several promoters that respond to different stimuli like light, anaerobiosis and hormones. It frequently regulates gene expression in synergy with other cis-elements.  Our research is aimed at understanding the mechanisms of transcriptional regulation, focusing on the role of cis-regulatory modules in regulation of gene expression. We did comparative genomic analysis of ACGT cis-regulatory elements across four different plant genomes. Our data indicated specific predominant spacer lengths between co-occurring ACGT elements, but these lengths were not universally conserved across all species under analysis. However, the frequency distribution indicated local regions of high correlation among monocots and dicots. Our data indicated specific predominant spacer lengths between co-occurring ACGT elements, but these lengths were not universally conserved across all species under analysis. However, the frequency distribution indicated local regions of high correlation among monocots and dicots. Sequence specificity data clearly revealed a preference for G at the first and C at the terminal position of a spacer sequence, suggesting that the G-box motif is the most prevalent for the ACGT class of promoters. Using gene expression databases, we also observed trends suggesting that co-occurring ACGT elements are responsible for gene regulation in response to exogenous stress. These findings were experimentally validated and the data obtained will be discussed.

Vljudno vabljeni! Seminar bo potekal v angleškem jeziku. / You are cordially invited to attend this lecture, which will be delivered in  English.

Dodatne informacije: doc. dr. Špela Baebler, spela.baebler@nib.si, Katja Sinur katja.sinur@nib.si.